Molecular cloning and preliminary characterization of estrogen receptor beta in Atlantic salmon
Introduction
We have cloned and sequenced an estrogen receptor (ER) from pituitary and testis of Atlantic salmon (Salmo salar) [1]. Sequence analyses revealed that the novel ER represented an estrogen receptor beta (ERβ) subtype. The cDNA sequence comprises 2685 nucleotides and the open reading frame encodes a protein of 594 amino acid residues. Results from yeast two-hybrid system indicate that ERβ has features like other nuclear receptors regarding protein dimerization. Expression of ERβ and ERα have been investigated by a semiquantitative PCR (qPCR) approach.
We have cloned and sequenced an estrogen receptor (ER) from pituitary and testis of Atlantic salmon (Salmo salar) [1]. Sequence analyses revealed that the novel ER represented an estrogen receptor beta (ERβ) subtype. The cDNA sequence comprises 2685 nucleotides and the open reading frame encodes a protein of 594 amino acid residues. Results from yeast two-hybrid system indicate that ERβ has features like other nuclear receptors regarding protein dimerization. Expression of ERβ and ERα have been investigated by a semiquantitative PCR (qPCR) approach.
Publisert i Nuclear Receptors, 2004
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